The PCR Basic Course is intended for participants without any previous knowledge in the field of PCR who plan to establish this technology in their labs. Therefore, in addition to the theoretical principles of PCR, we will focus on the different reaction components. Hands on experience will be provided in the set-up of several PCRs, product detection and data analysis following gel electrophoresis. Optimization of PCR conditions, primer design, and trouble shooting will also be a main part of the course. At the end of the seminar, you will be capable of performing a PCR assay and analyzing the data.
The theoretical part includes:
The practical part includes: